Biblioteca Digital do Instituto Politécnico de Bragança   Instituto Politécnico de Bragança

Biblioteca Digital do IPB >
Escola Superior Agrária >
Produção e Tecnologia Vegetal >
PTV - Artigos em Revistas Indexados ao ISI >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10198/3113

Título: Human cancer cell antiproliferative and antioxidant activities of Juglans regia L.
Autor: Carvalho, M.
Ferreira, P.J.
Mendes, V.S.
Silva, R.
Pereira, J.A.
Jerónimo, C.
Silva, B.M.
Palavras-chave: Juglans regia L.
Leaf
Green husk
Walnut
Antiproliferative activity
Antioxidant
Issue Date: 2010
Editora: Elsevier
Citação: Carvalho, M.; Ferreira, P.J.; Mendes, V.S.; Silva, R.; Pereira, J.A.; Jerónimo, C.; Silva, B.M. (2010) - Human cancer cell antiproliferative and antioxidant activities of Juglans regia L.. Food and Chemical Toxicology. ISSN 0278-6915. 48:1, p. 441-447
Resumo: Several studies suggest that regular consumption of nuts, mostly walnuts, may have beneficial effects against oxidative stress mediated diseases such as cardiovascular disease and cancer. Walnuts contain several phenolic compounds which are thought to contribute to their biological properties. The present study reports the total phenolic contents and antioxidant properties of methanolic and petroleum ether extracts obtained from walnut (Juglans regia L.) seed, green husk and leaf. The total phenolic contents were determined by the Folin–Ciocalteu method and the antioxidant activities assessed by the ability to quench the stable free radical 2,20-diphenyl-1-picrylhydrazyl (DPPH) and to inhibit the 2,20-azobis( 2-amidinopropane) dihydrochloride (AAPH)-induced oxidative hemolysis of human erythrocytes. Methanolic seed extract presented the highest total phenolic content (116 mg GAE/g of extract) and DPPH scavenging activity (EC50 of 0.143 mg/mL), followed by leaf and green husk. In petroleum ether extracts, antioxidant action was much lower or absent. Under the oxidative action of AAPH, all methanolic extracts significantly protected the erythrocyte membrane from hemolysis in a time- and concentration- dependent manner, although leaf extract inhibitory efficiency was much stronger (IC50 of 0.060 mg/ mL) than that observed for green husks and seeds (IC50 of 0.127 and 0.121 mg/mL, respectively). Walnut methanolic extracts were also assayed for their antiproliferative effectiveness using human renal cancer cell lines A-498 and 769-P and the colon cancer cell line Caco-2. All extracts showed concentrationdependent growth inhibition toward human kidney and colon cancer cells. Concerning A-498 renal cancer cells, all extracts exhibited similar growth inhibition activity (IC50 values between 0.226 and 0.291 mg/mL), while for both 769-P renal and Caco-2 colon cancer cells, walnut leaf extract showed a higher antiproliferative efficiency (IC50 values of 0.352 and 0.229 mg/mL, respectively) than green husk or seed extracts. The results obtained herein strongly indicate that walnut tree constitute an excellent source of effective natural antioxidants and chemopreventive agents.
Arbitragem científica: yes
URI: http://hdl.handle.net/10198/3113
ISSN: 0278-6915
Versão do Editor: http://www.sciencedirect.com/science/journal/02786915
Appears in Collections:PTV - Artigos em Revistas Indexados ao ISI

Files in This Item:

File Description SizeFormat
71.pdf650,45 kBAdobe PDFView/Open
Restrict Access. You can request a copy!
71 resumo.pdf440,14 kBAdobe PDFView/Open
Statistics
FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpaceOrkut
Formato BibTex mendeley Endnote Logotipo do DeGóis 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 


  © Instituto Politécnico de Bragança - Biblioteca Digital - Feedback - Statistics
  Estamos no RCAAP Governo Português separator Ministério da Educação e Ciência   Fundação para a Ciência e a Tecnologia

Financiado por:

POS_C UE