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Please use this identifier to cite or link to this item: http://hdl.handle.net/10198/3112

Title: Headspace solid-phase microextraction and gás chromatography/ion trap-mass spectrometry applied to a living system: Pieris brassicae fed with kale
Authors: Fernandes, F.
Pereira, D.M.
Pinho, P. Guedes de
Valentão, P.
Pereira, J.A.
Bento, Albino
Andrade, P.B.
Keywords: Brassica oleracea L. var. acephala
Kale
Pieris brassicae
Volatile compounds
HS-SPME
GC/IT-MS
Issue Date: 2009
Publisher: Elsevier
Citation: Fernandes, F.; Pereira, D.; Guedes de Pinho, P.; Valentão, P.; Pereira, J.A.; Bento, A.; Andrade, P.B. (2010) - Headspace solid-phase microextraction and gás chromatography/ion trap-mass spectrometry applied to a living system: Pieris brassicae fed with kale. Food Chemistry. ISSN 0308-8146. 119:4, p. 1681-1693
Abstract: The influence of Pieris brassicae feeding on kale was monitored, by evaluating its effect on the volatiles released by the plant through time. This is the first study applying headspace solid-phase microextraction (HS-SPME) and gas chromatography/ion trap-mass spectrometry to an isolated insect, as most studies analyse the insect–plant system as a whole, being unable to evaluate the contribution of the insect itself. Substantial differences were noticed between the volatiles composition of kale before and after the insect’s attack. More than 60 compounds were found, including terpenes, lipoxygenase pathway by-products, ketones, norisoprenoids, etc. After insect attack, l-camphor, sabinene and a-thujene were found and limonene and eucalyptol suffered a noticeable increase. A considerable rise in (Z)-3-hexenyl acetate was also observed. In vivo accumulation of limonene and camphor by the insect was detected. The findings contribute to the knowledge of the ecological interactions between the two species.
Peer Reviewed: yes
URI: http://hdl.handle.net/10198/3112
ISSN: 0308-8146
Publisher version: http://www.sciencedirect.com/science/journal/03088146
Appears in Collections:PTV - Artigos em Revistas Indexados ao ISI

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