Biblioteca Digital do Instituto Politécnico de Bragança   Instituto Politécnico de Bragança

Biblioteca Digital do IPB >
Escola Superior Agrária >
Ambiente e Recursos Naturais >
ARN - Artigos em Revistas Indexados ao ISI/Scopus >

Please use this identifier to cite or link to this item:

Título: Long term preservation of DNA from honey bees (Apis mellifera) collected in aerial pitfall traps
Autor: Rubink, William L.
Murray, K. D.
Baum, Kristen A.
Pinto, M. Alice
Palavras-chave: Honey bee
DNA preservation
Issue Date: 2003
Editora: Texas Academy of Science
Citação: Rubink, William L.; Murray, K. D.; Baum, Kristen A.; Pinto, M. Alice (2003) - Long term preservation of DNA from honey bees (Apis mellifera) collected in aerial pitfall traps. Texas Journal of Science. ISSN 0040-4403. 55:2, p. 159-168
Resumo: This study examines the preservation of nuclear and mitochondrial DNA from honey bee (Apis mellifera L.) specimens which were first kept in propylene glycol-based antifreeze under various conditions, and then stored long-term, refrigerated in 95% ethanol. Two sets of bees were subjected to the propylene glycol treatment, then ethanol storage. One set consisted of bees captured in the field in propylene glycol-containing "aerial pitfall traps", where they remained for up to 21 days. A second set consisted of bees taken from a hive and kept in propylene glycol under various temperature and lighting conditions for up to 90 days. Both the field bees and laboratory bees were then stored long-term in ethanol before evaluation of the persistence of nuclear and mitochondrial DNA using the polymerase chain reaction. DNA integrity was preserved for both field and laboratory specimens. The results demonstrate that propylene glycol-captured, ethanol-preserved honey bees retain both nuclear and mitochondrial DNA after capture and long tern preservation. It is suggested that with little or no modification, the techniques described here might he applied to other studies involving trap-collected arthropod specimens.
Arbitragem científica: yes
ISSN: 0040-4403
Appears in Collections:ARN - Artigos em Revistas Indexados ao ISI/Scopus

Files in This Item:

File Description SizeFormat
400.pdf2,45 MBAdobe PDFView/Open

Please give feedback about this item
FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpace
Formato BibTex MendeleyEndnote Logotipo do DeGóis 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.


© Instituto Politécnico de Bragança - Biblioteca Digital - Feedback - Statistics
Promotores do RCAAP   Financiadores do RCAAP

Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência PO Sociedade do Conhecimento (POSC) Portal oficial da União Europeia