Biblioteca Digital do Instituto Politécnico de Bragança   Instituto Politécnico de Bragança

Biblioteca Digital do IPB >
Escola Superior de Tecnologia e Gestão >
Tecnologia Química e Biológica >
DTQB - Artigos em Proceedings Não Indexados ao ISI/Scopus >

Please use this identifier to cite or link to this item:

Título: Decyl acetate synthesis by enzyme catalysis in sc-CO2
Autor: Rebocho, Sílvia F.
Ribeiro, Adriano S.
Ferreira, Olga
Oliveira, Manuela V.
Vidinha, Pedro
Barreiros, Susana
Macedo, Eugénia A.
Loureiro, José M.
Palavras-chave: Supercritical CO2
Immobilized lipase B of Candida antarctica
Ping-pong bi-bi mechanism
Decyl acetate
Issue Date: 2008
Editora: CHEMPOR
Citação: Rebocho, Sílvia F.; Ribeiro, Adriano S.; Ferreira, Olga; Oliveira, Manuela V.; Vidinha, Pedro; Barreiros, Susana; Macedo, Eugénia A.; Loureiro, José M. (2008) - Decyl acetate synthesis by enzyme catalysis in sc-CO2. In Proceedings of the 10th International Chemical and Biological Engineering Conference - CHEMPOR (CD-ROM). Braga. p.114-119. ISBN 978-972-97810-3-2
Resumo: The synthesis of decyl acetate, from the transesterification of vinyl acetate with decanol, was studied in a high-pressure experimental set-up, equipped with a variable volume batch reactor, operating isothermally at 35 ºC and 100 bar, using CO2 in supercritical conditions as solvent, and Candida antarctica lipase B (CALB), immobilized on the macroporous resin Lewatit B (Novozym 435®), as catalyst. The enzymatic content was determined for each particle size of the catalyst. It was shown that the smallest particles have a larger specific amount of enzyme, and the results indicate that the enzyme is located in an external shell of the particle, following an “egg-shell” model type, with a thickness of ca. 60 μm (assuming a homogeneous distribution), independent of the particle size. Both external and internal mass transfer resistances were evaluated. External resistances were easily eliminated by stirring and internal diffusional limitations can be considered as negligible. The effect of the feed concentration of substrates on the initial reaction rate was also studied. It was observed that, above a certain concentration of decanol in excess relatively to vinyl acetate, the reaction is inhibited by the alcohol. On the contrary, the reaction is favoured when the reactor is fed with excess of vinyl acetate, with a significant enhancement of its initial rate. These results are consistent with a Ping-pong bi-bi type mechanism with competitive inhibition by the alcohol, commonly used in the description of enzymatic reactions of esterification/ transesterification.
ISBN: 978-972-97810-3-2
Appears in Collections:DTQB - Artigos em Proceedings Não Indexados ao ISI/Scopus

Files in This Item:

File Description SizeFormat
Paper A24.pdf239,21 kBAdobe PDFView/Open
Restrict Access. You can request a copy!
Paper A24_BookAbstracts (1).pdf2,66 MBAdobe PDFView/Open

Please give feedback about this item
FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpace
Formato BibTex MendeleyEndnote Logotipo do DeGóis 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.


© Instituto Politécnico de Bragança - Biblioteca Digital - Feedback - Statistics
Promotores do RCAAP   Financiadores do RCAAP

Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência PO Sociedade do Conhecimento (POSC) Portal oficial da União Europeia