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Title: Tracking red blood cells in a circular PDMS microchannel using a confocal micro-PIV system
Authors: Lima, R.
Ishikawa, Takuji
Tanaka, S.
Takeda, Motohiro
Imai, Yohsuke
Tsubota, Ken-ichi
Wada, Shigeo
Yamaguchi, Takami
Keywords: Microcirculation
Red blood cells
Issue Date: 2007
Publisher: COE
Citation: Lima, R.; Ishikawa, T.; Tanaka, S.; Takeda, M.; Imai, Y.; Tsubota, K.; Wada, S.; Yamaguchi, T. (2007) - Tracking red blood cells in a circular PDMS microchannel using a confocal micro-PIV system. In 9th International Symposium on Future Medical Engineering based on Bio-nanotechnology. Sendai, Japan.
Abstract: The blood flow in microcirculation is characterized mainly by the flow of red blood cells (RBCs), which may be normal or pathological. This paper presents the application of a confocal micro-P1V system lo track RBCs through a circular polydimelhysiloxane (PDMS) microchannel. This technique, consists o!’ a spinning disk confocal microscope, high speed camera and a diode-pumped solid stale (DPSS) laser combined with a single particle tracking (SPT) software (Mtracki). To show the ability of this system detailed motions o!’ individual RBCs were measured at different haematocrits (Hct): 3%, 14% and 37%. Our results show clearly that this technique can provide detailed information about micro-scale disturbance effects caused by RBCs lo the blood flow.
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