Utilize este identificador para referenciar este registo: http://hdl.handle.net/10198/18028
Título: High sample throughput genotyping for estimating C-lineage introgression in the dark honeybee: an accurate and cost-effective SNP-based tool
Autor: Henriques, Dora
Browne, Keith A.
Barnett, Mark W.
Parejo, Melanie
Kryger, Per
Freeman, Tom. C.
Muñoz, Irene
Garnery, Lionel
Highet, Fiona
Jonhston, J. Spencer
McCormack, Grace P.
Pinto, M. Alice
Palavras-chave: Reduced SNP assays
Apis mellifera mellifera
Data: 2018
Citação: Henriques, Dora; Browne, Keith A.; Barnett, Mark W.; Parejo, Melanie; Kryger, Per; Freeman, Tom. C.; Muñoz, Irene; Garnery, Lionel; Highet, Fiona; Jonhston, J. Spencer; McCormack, Grace P.; Pinto, M. Alice (2018). High sample throughput genotyping for estimating C-lineage introgression in the dark honeybee: an accurate and cost-effective SNP-based tool. In EURBEE 2018: 8th European Conference of Apidology. Ghent, Belgium
Resumo: The natural distribution of the honeybee (Apis mellifera L.) has been changed by humans in recent decades to such an extent that the formerly widest-spread European subspecies, Apis mellifera mellifera, is threatened by extinction through introgression from highly divergent commercial strains in large tracts of its range. Conservation efforts for A. m. mellifera are underway in multiple European countries requiring reliable and cost-efficient molecular tools to identify purebred colonies. Here, we developed four ancestry-informative SNP assays for high sample throughput genotyping using the iPLEX Mass Array system. Our customized assays were tested on DNA from individual and pooled, haploid and diploid honeybee samples extracted from different tissues using a diverse range of protocols. The assays had a high genotyping success rate and yielded accurate genotypes. Performance assessed against whole-genome data showed that individual assays behaved well, although the most accurate introgression estimates were obtained for the four assays combined (117 SNPs). The best compromise between accuracy and genotyping costs was achieved when combining two assays (62 SNPs). We provide a ready-to-use cost-effective tool for accurate molecular identification and estimation of introgression levels to more effectively monitor and manage A. m. mellifera conservatories.
Peer review: yes
URI: http://hdl.handle.net/10198/18028
Aparece nas colecções:CIMO - Resumos em Proceedings Não Indexados à WoS/Scopus

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