Utilize este identificador para referenciar este registo: http://hdl.handle.net/10198/18025
Título: A novel SNP-based tool for estimating C-lineage introgression in the dark honey bee (Apis mellifera mellifera)
Autor: Pinto, M. Alice
Henriques, Dora
Browne, Keith A.
Barnett, Mark
Parejo, Melanie
Kryger, Per
Freeman, Tom C.
Muñoz, Irene
Garnery, Lionel
Highet, Fiona
Jonhston, J. Spencer
McCormack, Grace P.
Pinto, M. Alice
Palavras-chave: Dark bee
Apis mellifera mellifera
Reduced SNP assays
Data: 2018
Citação: Pinto, M. Alice; Henriques, Dora; Browne, Keith A.; Barnett, Mark; Parejo, Melanie; Kryger, Per; Freeman, Tom C.; Muñoz, Irene; Garnery, Lionel; Highet, Fiona; Jonhston, J. Spencer; McCormack, Grace P.; Pinto, M. Alice (2018). A novel SNP-based tool for estimating C-lineage introgression in the dark honey bee (Apis mellifera mellifera). In SICAMM 2018 Dark Bee Conference. Mustiala, Finland
Resumo: The natural distribution ofthe honeybee (Apis mellifera L.) hás been changed by humans in recent decades to such an extent that the formerly widest-spread European subspecies, Apís mellifera mellifera, is threatened by extinction through introgression from highly divergent commercial strains in large tracts of its range. Conservation efforts for A. m. mellifera are underway in multiple European countries requiring reliable and cost-efficient molecular tools to identify purebred colonies. Here, we developed four ancestry-informative SNP assays for high sample throughput genotyping using the iPLEX Mass Array system. Our customized assays were tested on DNA from individual and pooled, haploid and diploid honeybee samples extracted from different tissues using a diverse range of protocols. The assays had a high genotyping success rate and yielded accurate genotypes. Performance assessed against whole-genome data showed that individual assays behaved well, although the most accurate introgression estimates were obtained forthe fourassays combined (117 SNPs). The best compromise between accuracy ana genotyping costs was achieved when combining two assays (62 SNPs). We provide a ready-to-use cost-effective tool for accurate molecular identification and estimation of introgression leveis to more effectively monitor and manage A. m. mellífera conservatories.
Peer review: yes
URI: http://hdl.handle.net/10198/18025
Aparece nas colecções:CIMO - Resumos em Proceedings Não Indexados à WoS/Scopus

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