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Title: Quantification of tocopherols and tocotrienols in Portuguese olive oils using HPLC with three different detection systems
Authors: Cunha, S.C.
Amaral, J.S.
Fernandes, J.O.
Oliveira, M.B.P.P.
Keywords: Tocopherols
Olive oil
Issue Date: 2006
Publisher: ACS
Citation: Cunha, S.C.; Amaral, J.S.; Fernandes, J.O.; Oliveira, M.B.P.P. (2006) - Quantification of tocopherols and tocotrienols in Portuguese olive oils using HPLC with three different detection systems. Journal of Agricultural and Food Chemistry. ISSN 0021-8561. 54:9, p.3351–3356
Abstract: Three different HPLC detection systems were compared for the determination of tocopherols and tocotrienols in olive oil: fluorescence and diode array connected in series, ultraviolet, and evaporative light scattering. The best results were obtained with the fluorescence detector, which was successfully applied in the quantification of tocopherols and tocotrienols in 18 samples of Portuguese olive oils. To support the validity of the method, the parameters evaluated were linearity, detection limits, repeatability, and recovery. All of the studied samples showed similar qualitative profiles with six identified compounds: R-T, -T, γ-T, δ-T, R-T3, and γ-T3. R-Tocopherol (R-T) was the main vitamin E isomer in all samples ranging from 93 to 260 mg/kg. The total tocopherols and tocotrienols ranged from 100 to 270 mg/kg. Geographic origin did not seem to influence the tocopherol and tocotrienol composition of the olive oils under evaluation.
ISSN: 0021-8561
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Appears in Collections:DTQB - Artigos em Revistas Indexados ao ISI/Scopus

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