Utilize este identificador para referenciar este registo: http://hdl.handle.net/10198/1368
Título: In vitro biomineralization by osteoblast-like cells : Part II - characterisation of cellular culture supernatants
Autor: Morais, S.
Carvalho, G.S.
Faria, Joaquim
Gomes, Helder
Sousa, João
Palavras-chave: Cell culture medium
Microelectrodes
Spectroscopic techniques
Data: 1998
Editora: Elsevier
Citação: Morais, S.; Carvalho, G.; Faria, Joaquim; Gomes, Helder; Sousa, João (1998) - In vitro biomineralization by osteoblast-like cells : Part II - characterisation of cellular culture supernatants. Biomaterials. ISSN 0142-9612. 19:1-3, p. 23-29
Resumo: The quantification of total calcium, phosphorus, iron, chromium and nickel in cell culture medium by electrochemical or spectroscopic means may require digestion of samples. Nevertheless, when pH adjustment is performed for values higher than about 6.5, the formation of two phases occurs: a white precipitate and a clear solution. Analysing both phases using microelectrodes, atomic absorption spectrometry (AAS), diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy, X-ray dispersive (XRD) analysis, scanning electron microscopy (SEM) and energy dispersive spectroscopic (EDS) analysis, it was observed that iron, chromium and nickel are not co-precipitating with the white solid phase. If quantification of calcium, phosphorus and magnesium is intended, a ten-fold dilution at least, must be performed to avoid most of these elements going into the precipitate. This knowledge is crucial if a mineralization study is going to be made.
URI: http://hdl.handle.net/10198/1368
ISSN: 0142-9612
Versão do Editor: http://www.sciencedirect.com/science/journal/01429612
Aparece nas colecções:DTQB - Artigos em Revistas Indexados ao ISI/Scopus

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