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Title: In vitro biomineralization by osteoblast-like cells : Part II - characterisation of cellular culture supernatants
Authors: Morais, S.
Carvalho, G.S.
Faria, Joaquim
Gomes, Helder
Sousa, João
Keywords: Cell culture medium
Spectroscopic techniques
Issue Date: 1998
Publisher: Elsevier
Citation: Morais, S.; Carvalho, G.; Faria, Joaquim; Gomes, Helder; Sousa, João (1998) - In vitro biomineralization by osteoblast-like cells : Part II - characterisation of cellular culture supernatants. Biomaterials. ISSN 0142-9612. 19:1-3, p. 23-29
Abstract: The quantification of total calcium, phosphorus, iron, chromium and nickel in cell culture medium by electrochemical or spectroscopic means may require digestion of samples. Nevertheless, when pH adjustment is performed for values higher than about 6.5, the formation of two phases occurs: a white precipitate and a clear solution. Analysing both phases using microelectrodes, atomic absorption spectrometry (AAS), diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy, X-ray dispersive (XRD) analysis, scanning electron microscopy (SEM) and energy dispersive spectroscopic (EDS) analysis, it was observed that iron, chromium and nickel are not co-precipitating with the white solid phase. If quantification of calcium, phosphorus and magnesium is intended, a ten-fold dilution at least, must be performed to avoid most of these elements going into the precipitate. This knowledge is crucial if a mineralization study is going to be made.
ISSN: 0142-9612
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Appears in Collections:DTQB - Artigos em Revistas Indexados ao ISI/Scopus

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