Biblioteca Digital do Instituto Politécnico de Bragança   Instituto Politécnico de Bragança

Biblioteca Digital do IPB >
Escola Superior de Tecnologia e Gestão >
Tecnologia Química e Biológica >
DTQB - Artigos em Revistas Indexados ao ISI >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10198/1368

Título: In vitro biomineralization by osteoblast-like cells : Part II - characterisation of cellular culture supernatants
Autor: Morais, S.
Carvalho, G.S.
Faria, Joaquim
Gomes, Helder
Sousa, João
Palavras-chave: Cell culture medium
Microelectrodes
Spectroscopic techniques
Issue Date: 1998
Editora: Elsevier
Citação: Morais, S.; Carvalho, G.; Faria, Joaquim; Gomes, Helder; Sousa, João (1998) - In vitro biomineralization by osteoblast-like cells : Part II - characterisation of cellular culture supernatants. Biomaterials. ISSN 0142-9612. 19:1-3, p. 23-29
Resumo: The quantification of total calcium, phosphorus, iron, chromium and nickel in cell culture medium by electrochemical or spectroscopic means may require digestion of samples. Nevertheless, when pH adjustment is performed for values higher than about 6.5, the formation of two phases occurs: a white precipitate and a clear solution. Analysing both phases using microelectrodes, atomic absorption spectrometry (AAS), diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy, X-ray dispersive (XRD) analysis, scanning electron microscopy (SEM) and energy dispersive spectroscopic (EDS) analysis, it was observed that iron, chromium and nickel are not co-precipitating with the white solid phase. If quantification of calcium, phosphorus and magnesium is intended, a ten-fold dilution at least, must be performed to avoid most of these elements going into the precipitate. This knowledge is crucial if a mineralization study is going to be made.
URI: http://hdl.handle.net/10198/1368
ISSN: 0142-9612
Versão do Editor: http://www.sciencedirect.com/science/journal/01429612
Appears in Collections:DTQB - Artigos em Revistas Indexados ao ISI

Files in This Item:

File Description SizeFormat
Biomat-19-23-1998.pdf456,22 kBAdobe PDFView/Open
Biomat-19-23-1998_resumo.pdf50,13 kBAdobe PDFView/Open
Statistics
FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpaceOrkut
Formato BibTex mendeley Endnote Logotipo do DeGóis 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 


  © Instituto Politécnico de Bragança - Biblioteca Digital - Feedback - Statistics
  Estamos no RCAAP Governo Português separator Ministério da Educação e Ciência   Fundação para a Ciência e a Tecnologia

Financiado por:

POS_C UE