Utilize este identificador para referenciar este registo: http://hdl.handle.net/10198/13341
Título: Wild Roman chamomile extracts and phenolic compounds: enzymatic assays and molecular modelling studies with VEGFR-2 tyrosine kinase
Autor: Guimarães, Rafaela
Calhelha, Ricardo C.
Froufe, Hugo J.C.
Abreu, Rui M.V.
Carvalho, Ana Maria
Queiroz, Maria João R.P.
Ferreira, Isabel C.F.R.
Data: 2016
Editora: Royal Society of Chemistry
Citação: Guimarães, Rafaela; Calhelha, Ricardo C.; Froufe, Hugo J.C.; Abreu, Rui M.V.; Carvalho, Ana Maria; Queiroz, Maria João R.P.; Ferreira, Isabel C.F.R. (2016) - Wild Roman chamomile extracts and phenolic compounds: enzymatic assays and molecular modelling studies with VEGFR-2 tyrosine kinase. Food & Function. ISSN 2042-6496. 7:1, p. 79-83
Resumo: Angiogenesis is a process by which new blood vessels are formed from the pre-existing vasculature, and it is a key process that leads to tumour development. Some studies have recognized phenolic compounds as chemopreventive agents; flavonoids, in particular, seem to suppress the growth of tumor cells modifying the cell cycle. Herein, the antiangiogenic activity of Roman chamomile (Chamaemelum nobile L.) extracts (methanolic extract and infusion) and the main phenolic compounds present (apigenin, apigenin-7-O-glucoside, caffeic acid, chlorogenic acid, luteolin, and luteolin-7-O-glucoside) was evaluated through enzymatic assays using the tyrosine kinase intracellular domain of the Vascular Endothelium Growth Factor Receptor-2 (VEGFR-2), which is a transmembrane receptor expressed fundamentally in endothelial cells involved in angiogenesis, and molecular modelling studies. The methanolic extract showed a lower IC50 value (concentration that provided 50% of VEGFR-2 inhibition) than the infusion, 269 and 301 μg mL(-1), respectively. Regarding phenolic compounds, luteolin and apigenin showed the highest capacity to inhibit the phosphorylation of VEGFR-2, leading us to believe that these compounds are involved in the activity revealed by the methanolic extract.
Peer review: yes
URI: http://hdl.handle.net/10198/13341
DOI: 10.1039/c5fo00586h
ISSN: 2042-6496
Aparece nas colecções:CIMO - Artigos em Revistas Indexados à WoS/Scopus

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