Utilize este identificador para referenciar este registo: http://hdl.handle.net/10198/10492
Título: A new effective assay to detect antimicrobial activity of filamentous fungi
Autor: Pereira, Eric Carvalho
Santos, A.
Reis, Francisca
Tavares, Rui Manuel
Baptista, Paula
Lino-Neto, Teresa
Almeida-Aguiar, Cristina
Palavras-chave: Antimicrobial assay
Anti-yeast activity
Filamentous fungus
Microbiological method
Data: 2013
Editora: Elsevier
Citação: Pereira, Eric Carvalho; Santos A.; Reis, Francisca; Tavares, Rui Manuel; Baptista, Paula; Lino-Neto, Teresa; Almeida-Aguiar, Cristina (2013). A new effective assay to detect antimicrobial activity of filamentous fungi. Microbiological Research. ISSN 0944-5013. 168:1, p. 1-5
Resumo: The search for new antimicrobial compounds and the optimization of production methods turn the use of antimicrobial susceptibility tests a routine. The most frequently used methods are based on agar diffusion assays or on dilution in agar or broth. For filamentous fungi, the most common antimicrobial activity detection methods comprise the co-culture of two filamentous fungal strains or the use of fungal extracts to test against single-cell microorganisms. Here we report a rapid, effective and reproducible assay to detect fungal antimicrobial activity against single-cell microorganisms. This method allows an easy way of performing a fast antimicrobial screening of actively growing fungi directly against yeast. Because it makes use of an actively growing mycelium, this bioassay also provides a way for studying the production dynamics of antimicrobial compounds by filamentous fungi. The proposed assay is less time consuming and introduces the innovation of allowing the direct detection of fungal antimicrobial properties against single cell microorganisms without the prior isolation of the active substance(s). This is particularly useful when performing large screenings for fungal antimicrobial activity. With this bioassay, antimicrobial activity of Hypholoma fasciculare against yeast species was observed for the first time.
Peer review: yes
URI: http://hdl.handle.net/10198/10492
DOI: 10.1016/j.micres.2012.06.008
ISSN: 0944-5013
Aparece nas colecções:CIMO - Artigos em Revistas Indexados à WoS/Scopus

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