Advisor(s)
Abstract(s)
Total phenolic and flavonoids phytochemical concentration was measured in bee pollen samples,
Appis Mellifera , as well as their botanical origin, antioxidant activity and antimicrobial activity.
Pollen loads were washed with 70% ethanol and identified using a 400x magnification. The content of
total polyphenols and flavonoids was measured spectrophotometrically with gallic acid and quercetin
as standard. The antioxidant activity was evaluated by the linking capacity of (i)the free radical DPPH
(2,2-Diphenyl-1-picrylhydrazyl), (ii)ORAC and (iii)system β-carotene/linoleic acid. The experiments
were performed in triplicate and the results expressed as mean ± standard deviation. All statistical
analysis were performed using the program STATISTICA 8.0 and adopting the significance level of
5% (p<0.05). Were using six samples of dehydrated bee pollen collected in Ribeirao Preto, SP, Brazil
during the years of 2010 and 2011. Five families were found in the mixture of bee pollen: Mimosa
caesalpiniaefolia , Mimosa scabrella, Asteraceae, Poaceae, Zea mays. Each dried bee pollen sample
was composed mainly of Mimosa caesalpiniaefolia (Fabaceae-Mimosoideae). The variations were
(dry basis): 17.64±1.57 to 32.14±2,88 mg GAE/g of bee pollen for phenolic compounds; 2.51±0.28 to
3.71±0.25 mg quercetin/g of pollen for flavonoids concentration; 77.15±0.72 to 90.56% for antioxidant
activity (DPPH) and 3.19±0.25 to 4.61±0.33 mg/mL for EC50; 183.42±16.26 to 338.32± 33.38
μmols eq. Trolox/g for ORAC and 68.96±2.75 to 85.90±2.27% for β -carotene/linoleic acid. Samples
of pollen, phenolics and flavonoids have the potential biological, showing high antioxidant activity by
the three methods used. It was verified that the presence of pollen differentially affected the growth of
bacteria Gram-positive, Gram-negative and yeasts under study, depending this on the microorganism
and the pollen used in fact, only one after sample was monofloral Mimosa caesalpiniaefolia. This is
an important study since; their characterization can increase their economic value.
The opinions, hypothesis and conclusions or recommendations expressed in the article are those
of the authors and do not necessarily coincide with those of FAPESP.
Description
Keywords
Bee pollen Antioxidant activity Antimicrobial activity Appis Mellifera Bee products
Citation
Arruda, Vanilda Aparecida Soares de; Santos, Alexandre Vieira dos; Figueiredo, Davi; Meira, Sampaio; Estevinho, Leticia M.; Barth, Ortrud Monika; Freitas, Alex da Silva de; Almeida-Muradian, Ligia Bicudo de (2012). Brazilian samples of bee samples pollen: Palynological origin, phenolic content, antioxidant properties and antimicrobial activity. In X Encontro sobre Abelhas. Ribeirão Preto, Brasil