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Advisor(s)
Abstract(s)
Historically, extracts and preparations of plants are the basis of traditional medicine and the starting point for the discovery of new therapeutic agents. Cytisus multiflorus is used in folk medicine and it is claimed to have various health benefits, including anti-inflammatory properties. Still, no scientific data regarding this ability has been described for this plant. The present work aims to clarify the antioxidant capacity and the anti-inflammatory mechanisms of Cytisus multiflorus.
A purified ethanolic extract was prepared and its antioxidant abilities were evaluated through the DPPH scavenging and reducing power assays. Anti-inflammatory properties were assessed by determining the amounts of nitric oxide (NO) on a chemical and on lipopolysaccharide-stimulated Raw 264.7 macrophages models. Furthermore, the effects on two proteins that are potential targets to prevent or treat chronic inflammation, namely cyclooxygenase-2 (COX-2) and inducible NO synthase (iNOS), were also estimated in the cellular model by Western Blot analysis.
The ethanolic extract showed high antioxidant capacity, with EC50 values of 13.4±1.0 and 11.4±2.1 μg/mL for DPPH and reducing power assays, respectively. It also efficiently scavenged the NO radical and inhibited the NO production, in the chemical and cellular models, respectively. Furthermore, despite no changes on intracellular COX-2 levels were observed, iNOS expression was significantly diminished by the treatment with non-toxic concentrations of Cytisus multiflorus ethanolic extract.
The present results suggest that Cytisus multiflorus is a good antioxidant and that it actually exerts an anti-inflammatory action.
Description
Keywords
Cytisus multiflorus Antioxidant activity NO radical scavenging iNOS expression
Citation
Saraiva, Susana C.; Pereira, Olívia R.; Liberal, Joana; Batista, Maria T.; Cruz, Maria T.; Cardoso, Susana M. ( 2013). NO radical scavenging and iNOS expression inhibition by cytisus multiflorus. In 47th Annual Scientific Meeting of the European Society for Clinical Investigation. Albufeira. p. 62. ISSN 0014-2972
Publisher
Wiley-Blackwell