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Abstract(s)
Hoje em dia na literatura está disponível uma grande quantidade de informação sobre os
aspetos químicos e biológicos dos produtos apícolas, no entanto a informação científica
fundamentada sobre a sua utilização terapêutica é limitada. O objetivo deste estudo foi
avaliar o perfil fenólico, a atividade antimicrobiana in vitro e o efeito sobre a enzima
hialuronidase (amplamente relacionado com o processo de inflamação) do própolis
Português. Foi também comparada a eficácia da extração do própolis em três extractos
(hidro-alcoólico, metanólico e aquoso). Foi escolhido o extrato hidro-alcoólico, porque
foi o mais eficaz na extração de fenóis totais.
Foi analisada a atividade antimicrobiana do própolis contem bactérias Gram-positivas e
Gram-negativas e leveduras, isoladas de diferentes fluídos biológicos. Os resultados
foram comparados com os obtidos para microrganismos de referência. O própolis de
Bragança foi o que possuiu o mais alto teor de fenóis totais. A amostra de Beja
evidenciou a inibição menos significativa da enzima hialuronidase.
Em relação à atividade antimicrobiana, Candida albicans foi a mais resistente e
Staphylococcus aureus a mais sensível. Os micorganismos de coleção foram mais
sensíveis do que os isolados a partir de fluídos biológicos.
Nowadays a great amount of information regarding chemical and biological aspects of bee products is available in the literature, but few data on their therapeutic uses are found. The aim of this study was to evaluate the phenolic profile, the in vitro antimicrobial activity and effect in the hyaluronidase enzyme (widely related with the inflammation process) of propolis harvested in Portugal. The efficacy of three extracts (hydro-alcoholic, methanolic and aqueous) was also compared. It was chosen the hydroalcoholic extract, because this was the most effective for extracting phenolic compounds. The antimicrobial activity was accessed in Gram-positive and Gramnegative bacteria and yeasts, isolated from different biological fluids and the results were then compared with the obtained for reference microorganisms. The propolis from Bragança was the one that possessed the highest polyphenols’ content. The sample from Beja showed less significant inhibition of the hyaluronidase enzyme. Concerning the antimicrobial activity, Candida albicans was the most resistant and Staphylococcus aureus the most sensitive microganism. The reference microorganisms were more sensitive than the ones isolated from biological fluids.
Nowadays a great amount of information regarding chemical and biological aspects of bee products is available in the literature, but few data on their therapeutic uses are found. The aim of this study was to evaluate the phenolic profile, the in vitro antimicrobial activity and effect in the hyaluronidase enzyme (widely related with the inflammation process) of propolis harvested in Portugal. The efficacy of three extracts (hydro-alcoholic, methanolic and aqueous) was also compared. It was chosen the hydroalcoholic extract, because this was the most effective for extracting phenolic compounds. The antimicrobial activity was accessed in Gram-positive and Gramnegative bacteria and yeasts, isolated from different biological fluids and the results were then compared with the obtained for reference microorganisms. The propolis from Bragança was the one that possessed the highest polyphenols’ content. The sample from Beja showed less significant inhibition of the hyaluronidase enzyme. Concerning the antimicrobial activity, Candida albicans was the most resistant and Staphylococcus aureus the most sensitive microganism. The reference microorganisms were more sensitive than the ones isolated from biological fluids.
Description
Keywords
Atividade antimicrobiana Hialuronidase Inflamação Compostos fenólicos Própolis