Optimization of the determination of tocopherols in Agaricus sp. edible mushrooms by a normal phase liquid chromatographic method

Barros, Lillian; Correia, Daniela M.; Ferreira, Isabel C.F.R.; Baptista, Paula; Santos-Buelga, Celestino

http://hdl.handle.net/10198/838

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Authors

Barros, Lillian

Correia, Daniela M.

Ferreira, Isabel C.F.R.

Baptista, Paula

Santos-Buelga, Celestino

Abstract(s)

Individual tocopherol profile of five Agaricus mushroom species, widely consumed in Portugal, was obtained by a normal-phase high-performance liquid chromatography (NP-HPLC). It was used a simple solid–liquid extraction procedure without saponification step and the chromatographic separation was achieved using a YMC-Pack Polyamine II column using an isocratic elution with hexane/ethyl acetate (70:30, v/v) at a flow rate of 1.0 mL/min. The effluent was monitored by a fluorescence detector. All the compounds were separated in a period of time of 30 min. The method proved to be sensitive, reproducible and accurate, allowing the determination of tocopherols.