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Advisor(s)
Abstract(s)
The present study investigates the phenolic composition and the antioxidant capacity of an ethanolic extract from flowers of C. multiflorus. As determined by HPLC-DAD, ESI-MSn and NMR combined analysis, this extract was mainly composed of chrysin-7-O-β-D-glycopyranoside, and also contained considerable amounts of rutin, a dihydroxyflavone isomer of chrysin and glycosidic derivatives of luteolin and apigenin. The C. multiflorus ethanolic extract exhibited high DPPH scavenging activity and reducing power with EC50 of 13.4 ± 0.6 and 95.7 ± 4.6 µg/mL, respectively. The exposure of human hepatoblastoma HepG2 cells to the non-toxic concentrations of the extract (50 or 200 µg/mL) resulted in a decreased rate of ROS production in a concentration dependent manner. Moreover, the mixture of standards that simulated the phenolic composition of the plant afforded a protection of about 50% in intracellular ROS production. Hence, the gathered results suggest that C. multiflorus polyphenols are closely associated to its antioxidant properties.
Description
Keywords
Cytisus multiflorus Phenolic compounds Antioxidant activity
Citation
Pereira, Olívia R.; Perez, Maria J.; Macias, Rocio I. R.; Domingues, Maria R. M.; Silva, Artur M. S.; Marin, Jose J. G.; Cardoso, Susana M. (2012). Cytisus multiflorus: source of antioxidant polyphenols. In 11º Encontro Química dos Alimentos. Bragança
Publisher
Instituto Politécnico de Bragança