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Advisor(s)
Abstract(s)
Crataegus monogyna has been extensively studied due to its various alleged health benefits. This study aimed
to determine the human tumor cells growth inhibitory activity of phenolic extracts of its flower buds and
fruits in three phenological stages, and further characterize the extracts by HPLC–DAD–ESI/MS. Flower bud
extract showed the highest antiproliferative activity as indicated by the lowest GI50 values obtained in all
the tested cell lines: MCF-7, breast adenocarcinoma; NCI-H460, non-small cell lung cancer; HeLa, cervical
carcinoma; HepG2, hepatocellular carcinoma. Furthermore, porcine liver primary cell culture (PLP2) was
used to evaluate toxicity to non-tumor cells. Flavonoids, particularly flavonols and flavones (higher in
flower buds) and proanthocyanidins (higher in unripe fruits) were the main classes in the studied
samples. Phenolic acids (mainly hydroxycinnamic acid derivatives) were also detected in significant
amounts, especially in flower bud extract. Regarding anthocyanins, over ripened fruits gave the highest
content. The higher bioactivity observed in flower buds might be related with its higher content in
phenolic compounds.
Description
Keywords
Crataegus monogyna Phenolic profiles Human tumor cell lines Antiproliferative activity
Pedagogical Context
Citation
Rodrigues, Sandra; Calhelha, Ricardo C.; Barreira, João C.M.; Dueñas, Montserrat; Carvalho, Ana Maria; Abreu, Rui M.V.; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R. (2012). Crataegus monogyna buds and fruits phenolic extracts: growth inhibitory activity on human tumor cell lines and chemical characterization by HPLC–DAD–ESI/MS. Food Research International. ISSN 0963-9969. 49:1, p. 516-523
Publisher
Elsevier