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Abstract(s)
Coriander is commonly used for medicinal purposes, food applications, cosmetics and
perfumes. It is a source of a variety of polyphenols and other phytochemicals, related to
its high antioxidant activity. The plant cell culture technique was applied to the production
of coriander, and a detailed analysis of lipophilic and hydrophilic antioxidants was carried
out in in vivo and in vitro grown samples. In vivo grown samples were vegetative parts and
seeds obtained in a local supermarket. In vitro grown samples were two clones
differentiated after 6 months in culture: clone A with a notorious purple pigmentation on
vegetative parts and clone B -just green (1,2]. Tocopherols, sugars and phenolic
compounds (phenolic acids, flavonoids and anthocyanins) were identified and quantified
by HPLC coupled to different detectors (fluorescence, RI, DAD and MS). The antioxidant
activity was also evaluated by free radical scavenging activity, reducing power and lipid
peroxidation inhibition in brain cells homogenates. The in vivo vegetative parts showed
the highest antioxidant activity mainly due to its highest levels of hydrophilic compounds;
quercetin-3-0-rutinoside was the main polyphenol. In vivo seeds revealed only phenolic
acids and derivatives. In vitro samples also gave promising antioxidant activity and a high
diversity of polyphenols, being C-glycosylated apigenin the main compound. Anthocyanins
were only found in clone A, which is certainly related to its purple pigmentation. In vitro
samples, mainly clone A, gave the highest concentration in lipophilic compounds but a
different profile when compared to the in vivo sample. Clones A and B revealed a lack of
~- and cS-tocopherols, a decrease in a-tocopherol, and an increase in y-tocopherol. In vitro
culture might be useful to explore the plants potentialities for industrial applications,
controlling growth conditions to produce bioactive products.
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Citation
Dias, Maria Inês; Barros, Lillian; Sousa, Maria João; Ferreira, Isabel C.F.R. (2012). Lipophilic and hydrophilic antioxidants from in vivo and in vitro grown Coriandrum sativum L.: chromatographic analysis. In 7º Encontro Nacional de Cromatografia. Porto