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Advisor(s)
Abstract(s)
A species-specific duplex polymerase chain reaction (PCR) assay was developed for the simultaneous
detection of pork and poultry meat species using the mitochondrial cytb and 12S rRNA as target genes
for pork and poultry, respectively. By the amplification of binary reference meat mixtures, a linear normalised
calibration curve was obtained using the fluorescence intensities of PCR products for pork
(149 bp) and poultry (183 bp) species. The proposed method allowed the quantification of pork meat
addition to poultry meat in the range of 1â75%, with a sensitivity of 0.1%. The in-house validation using
samples with known amounts of pork meat (1.0%, 2.5%, 7.5%, 20.0% and 40%) evidenced a high reproducibility
of the methodology (coefficient of variation from 4.1% to 7.6%). The successful application of the
duplex PCR was also demonstrated by the high correlation (R2 = 0.99) obtained from regression analysis
between the predicted and the actual values of pork meat addition in blind meat mixtures. The suggested
methodology presents a low cost, fast, easy and reliable alternative to estimate the level of poultry meat
adulteration by the addition of pork meat.
Description
Keywords
Duplex PCR Species identification Pork Poultry Meat Authenticity
Citation
Soares, S.; Amaral, J.S.; Mafra, I.; Oliveira, M.B.P.P. (2010). Quantitative detection of poultry meat adulteration with pork by a duplex PCR assay. Meat Science. ISSN 0309-1740. 85:3, p. 531-536
Publisher
Meat Science