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Advisor(s)
Abstract(s)
This study reports for the first time the biological properties of Portuguese propolis. The antioxidant
potential of propolis samples from Bornes (Northeast) and Fundão (Centre) regions of Portugal was evaluated
by their ability to inhibit the 2,20-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced oxidative
hemolysis and lipid peroxidation in human erythrocytes. Bornes and Fundão propolis strongly
protected the erythrocyte membrane from hemolysis (IC50 of 6.3 ± 0.7 and 10.4 ± 2.7 lg/ml, respectively),
in a time- and concentration-dependent manner. This effect was found to be significantly higher than
that presented by ascorbic acid (IC50 of 31.0 ± 5.6 lg/ml). In addition, human erythrocytes treated with
propolis extracts showed concentration-dependent decrease in levels of malondialdehyde, a breakdown
product of lipid peroxidation. Propolis extracts were also assayed for their anticancer properties on
human renal cell carcinoma (RCC). Primary cultures of normal and cancerous renal cells derived from
RCC patients, in addition to A-498 cell line, were treated with propolis extracts (0–100 lg/ml). Cytotoxic
and antiproliferative effects were determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide (MTT) assay. Propolis extracts exhibited selective toxicity against malignant cells compared
to normal cells. In vitro RCC growth was strongly inhibited by Bornes and Fundão propolis in a concentration-
dependent manner. Our results indicate that Portuguese propolis constitutes an excellent source
of effective natural antioxidant and chemopreventive agents.
Description
Keywords
Propolis Cancer chemoprevention
Citation
Valente, Maria J.; Baltazar, Ana F.; Henrique, Rui; Estevinho, Leticia M.; Carvalho, Márcia (2011). Biological activities of Portuguese propolis: protection against free radical-induced erythrocyte damage and inhibition of human renal cancer cell growth in vitro. Food and Chemical Toxicology. ISSN 0278-6915. 49:1, p. 86-92
Publisher
Elsevier