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Advisor(s)
Abstract(s)
In the present study, three different methods
were used to identify yeast isolated from Trás-os-Montes, Portuguese honey. A total of 24 isolates
were identified using a partial sequence of the 26S
rRNA gene (rDNA), restriction patterns generated
from the region spanning the internal transcribed
spacers (ITS1 and ITS2) of the 5.8S rRNA gene
and the API 20C AUX kit. Nine different yeast
species were identified representing six different
genera. Among the isolated honey samples,
Rhodotorula mucilaginosa, Candida magnoliae
and Zygosaccharomyces mellis were the
predominant species. Partial sequence of the 26S
rDNA yielded the best results in terms of correct
identification, followed by-5.8S-ITS analysis. The
commercial identification kit API 20C AUX was
able to correctly identify only 58% of the isolates.
Two new 5.8S-ITS profiles were described,
corresponding to Trichosporon mucoides and
Candida sorbosivorans.
Description
Keywords
Honey yeast identification 26S rDNA gene
Citation
Carvalho, Marisa; Meirinhos, Sofia; Estevinho, Leticia M.; Choupina, Altino (2010). Yeast species associated with honey: different identification methods. Archivos de Zootecnia. ISSN: 0004-0592. 59:225, p. 103-113
Publisher
Instituto De Zootecnica