Advisor(s)
Abstract(s)
Pollen analysis, total phenols content and antioxidant activity were studied for the first time in Portuguese
propolis samples from Bornes and Fundão regions. Total phenols content was determined by colorimetric
assay and their amount was of 329 mg/g of GAE in Bornes sample and 151 mg/g of GAE in Fundão propolis.
The antioxidant capacity of propolis extracts was assessed through the scavenging effects on DPPH (2,2-
diphenyl-1-picrylhydrazyl) and reducing power of iron (III)/ ferricyanide complex assays. A concentration-
dependent antioxidative capacity was verified in DPPH and reducing power assays. Low values of
EC50 on DPPH scavenging assay were obtained for Bornes and Fundão propolis (of 6.22 lg/mL and
52.00 lg/mL, respectively). For reducing power the values were 9.00 lg/mL, for Bornes propolis, and
55.00 lg/mL, for Fundão propolis. The high activity of propolis from Bornes could be related with their different
pollen composition. The results obtained indicate that Portuguese propolis is an important source of
total phenols showing antioxidant properties that could be beneficial for human health.
Description
Keywords
Propolis Polinic analysis
Citation
Moreira, Leandro; Dias, L.G.; Pereira, J.A.; Estevinho, Letícia M. (2008). Antioxidant properties, total phenols and pollen analysis of propolis samples from Portugal. Food and Chemical Toxicology. ISSN 0278-6915. 46:11, p. 3482- 3485
Publisher
Elsevier