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Advisor(s)
Abstract(s)
In aerobic organisms, the free radicals are constantly being produced during the normal
cellular metabolism. The antioxidant properties of many organisms and particularly of wild mushrooms with their content in antioxidant compounds such as tocopherols, can detoxify potentially damaging forms of activated oxygen. Herein, a comparative study of tocopherols composition and antioxidant properties of in vivo (fruiting bodies) and in vitro (mycelia) ectomycorrhizal fungi: Paxillus involutus and Pisolithus arhizus. Tocopherols were determined by high performance liquid chromatography (HPLC)
coupled to a fluorescence detector. The antioxidant properties were studied in terms of DPPH radical-scavenging activity, reducing power and inhibition of β-carotene
bleaching. Fruiting bodies revealed the highest antioxidant properties, including
scavenging effects on free radicals (EC50 = 0.61 and 0.56 mg/ml) and inhibition of lipid
peroxidation capacity (EC50 = 0.40 and 0.24 mg/ml for Paxillus involutus and Pisolithus
arhizus, respectively), than mycelia produced in vitro cultures. Nevertheless, mycelia revealed higher levels of total tocopherols than fruiting bodies, and particularly Pisolithus arhizus mycelium proved to be a powerful source of γ-tocopherol (154.39 μg/g dry weight).
Description
Keywords
Paxillus involutus Pisolithus arhizus Fruiting bodies/mycelia Tocopherols HPLC-fluorescence Antioxidant activity
Pedagogical Context
Citation
Reis, Filipa S.; Ferreira, Isabel C.F.R.; Barros, Lillian; Martins, Anabela (2011). A comparative study of tocopherols composition and antioxidant properties of in vivo and in vitro ectomycorrhizal fungi. LWT - Food Science and Technology. ISSN 0023-6438. 44:4, p. 820-824
Publisher
Elsevier