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Advisor(s)
Abstract(s)
ln this study, the antioxidant properties 01 different chestnut constituents (1Iowers,
leaves, skins and fruits) were evaluated through several biochemical assays: DPPH (2 ,2-
diphenyl-1-picrylhydrazyl) radical scavenging activity, reducing power, inhibition 01 ~carotene
bleaching, inhibition 01 oxidative hemolysis in erythrocytes, induced by 2,2'azobis(
2-amidinopropane)dihydrochloride (AAPH), and inhibition 01 lipid peroxidation in
pig brain tissue through formation 01 thiobarbituric acid reactive substances (TBARS).
These assays have been extensively studied as models for the peroxidative damage in
biomembranes. For all the methods EC50 values were calculated in order to evaluate the
antioxidant efficiency 01 each product. The phenol and flavonoid contents were also
obtained and correlated to antioxidant activity. Chestnut skins revealed much better
antioxidant properties, presenting much lower EC50 values, particularly lor lipid
peroxidation inhibition in TBAR8 assay. Also, the highest antioxidant contents (phenols
and flavonoids) were found for this constituent.
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Pedagogical Context
Citation
Barreira, João C.M.; Ferreira, Isabel C.F.R.; Oliveira, M. Beatriz P.P.; Pereira, José Alberto (2007). Antioxidant activity of chestnut constituents: flowers, leaves, skins and fruits. In II Congresso Ibérico do Castanheiro. Vila Real