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Advisor(s)
Abstract(s)
ln order to promote the use of mushrooms as a source of nutraceuticals, severa! experiments were performed in Cantharelfus cibarius. Hypholoma fasciculare,
Lepista nuda, Lycoperdon molle, Lycoperdon perlatum, Ramaria botrytis and
Tricholoma acerbum wild species. Their antioxidant activity was screened
through chemical and biochemical assays; chemical assays allowed an
evaluation of their reducing power, radical scavenging activity and inhibition of
P-carotene bleaching capacity, while biochemical assays evaluated the lipid
peroxidation inhibition capacity, using brain cells as models. The analysis of
nutraceuticals included determination of fatty acids by GC-FID, and other
phytochemicals such as tocopherols, by HPLC-fluorescence, and phenolics,
flavonoids, carotenoids and ascorbic acid, by spectrophotometer techniques. Ali
the species proved to have antioxidant activity, being R. botrytis the most
effective mushroom (EC50 values lower than I mg/mL). These properties seem
to be related to its higher content in phenols and tocopherols. lt also presented
the highest MUFA (particularly oleie acid) and the lowest SFA levels, which
m ay be relevant since the substitution of SFA with MUFA leads to an increase
in HDL cholesterol and decrease in LDL cholesterol, triacylglycerol, lip id
oxidation, and LDL susceptibility to oxidation. This study contributes not only
to a better knowledge of mushrooms but also to its valorisation.
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Citation
Barros, Lillian; Baptista, Paula; Ferreira, Isabel C.F.R. (2008). Mushrooms as a source of nutraceuticals related to antioxidant properties. In XVI Congresso Nacional de Bioquímica. Ponta Delgada