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Advisor(s)
Abstract(s)
The detail measurements of velocity profiles of in vitro blood
flow in micorchannels are fundamental for a better understanding
on the biomechanics of the microcirculation. Despite the high
amount of research in microcirculation, there is not yet any
detailed experimental information about flow velocity profiles,
RBCs deformability and aggregation in microvessels (diameter in
the order of 100μm or less). These lack of knowledge is mainly
due to the absence of adequate techniques to measure and
quantitatively evaluate fluid mechanical effects at a microscopic
level [1, 2].
During the years the most research work in this area has focused
in experimental studies using techniques such as laser Doppler
anemometry (LDA) or conventional particle image velocimetry
(PIV). However, due to limitations of those techniques to study
effects at a micro-scale level, Meinhart and his colleagues [3] have
proposed a measurement technique that combines the PIV system
with an inverted epi-fluorescent microscope, which increases the
resolution of the conventional PIV systems [3]. More recently,
considerable progress in the development of confocal microscopy
and consequent advantages of this microscope over the
conventional microscopes [4, 5] have led to a new technique
known as confocal micro-PIV. This technique combines the
conventional PIV system with a spinning disk confocal
microscope (SDCM). Due to its outstanding spatial filtering
technique together with the multiple point light illumination
system, this kind of microscope has the ability to obtain in-focus
images with optical thickness less than 1 μm, task extremely
difficult to be achieved by using a conventional microscope. As a
result, by combining SDCM with the conventional PIV system it
is possible to achieve a PIV system with not only extremely high
spatial resolution but also with capability to generate 3D velocity
profiles.
The main purpose of the present study is to evaluate the
performance of our confocal micro-PIV system in order to
investigate its ability to study the behaviour of non-homogenous
fluids such as physiological fluids.
Description
Keywords
Red blood cell Confocal micro-PIV Microchannel Microcirculation
Citation
Lima, R.; Wada, S.; Tsubota, K.; Yamaguchi, T. (2005). Velocity measurements of physiological flows in microchannels using a confocal micro-PIV system. In 16th JSME Conference on Frontiers in Bioengineering. Kusatsu, Japan.