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Advisor(s)
Abstract(s)
The detail measurements of velocity profiles of blood flow in microchannels
are fundamental for a better understanding on the biomechanics of the
microcirculation. It is therefore very important to obtain measurements with
high accuracy and spatial resolution of the influence of the blood cells on
the plasma flow behaviour. This paper presents and compares measurements
of in vitro blood with different hematocrits within a square microchannel
obtained by a confocal particle image velocimetry (PIV) system. This emerging
technology by combining the conventional PIV system with a spinning confocal
microscope has the ability to obtain not only high spatial resolution images but
also three-dimensional (3D) optical sectioning velocity measurements. Velocity
measurements of plasma seeded with 1 ~tm diameter fluorescent particles were
performed at different locations along the depth of 100 ~tm square microchannel
at a constant flow rate (0.15~tl/min) and Reynolds number (Re) of 0.025. By
using our confocal micro-PIV system, it was possible to obtain time-series of
instantaneous velocity profiles with high spatial resolution of 28.24 18.83 ~tm
at time intervals of 5 ms between two images. The ensemble-averaged velocity
results of blood flow with different hematocrits (up to 25%) have shown velocity
profiles very close to a parabolic shape. However, by analysing the temporal
variance of the instantaneous velocity profiles of different hematocrits, we
have observed a substantial increase of the instantaneous velocity fluctuations
by increasing the hematocrit within the plasma flow. Besides, some possible
effects from the measurements accuracy and flow rate instabilities from the
syringe pump, this observation also suggests that there is a direct correlation
between the level of hematocrit and the temporal instantaneous velocity
fluctuations.
Description
Keywords
Red blood cell Confocal micro-PIV Microchannel
Citation
Lima, R.; Wada, S.; Tsubota, K.; Yamaguchi, T. (2006). Confocal micro-PIV measurements of blood flow in microchannels. Journal of Biomechanics. ISSN 0021-9290. 39:Supplement 1, p.S332