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Advisor(s)
Abstract(s)
The objective of this study was to determinate some microbiological parameters of seven dehydrated
bee-pollen collected from September to December of2012, in Brazil, before and after irradiation, in order
to observe if the conservation process influenced the final microbiological load. All bee-pollen samples
were collected in the same apiary and processed as usual; then one parcel of each one was gamma-irradiated
to achieve dose of 5.0 kGy with a cobalt-60 source (by EMBRARAD/Empresa Brasileira de Radia~aes
Ltda, Cotia, SAo Paulo, Brasil). The bee-pollen samples were analysed to enumerate aerobic mesophilic
and psychotrophs bacteria, Staphylococcus aureus, total coliforms and yeasts and molds and to determine
the presence or absent of sulphite-reducing Clostridium spores and Salmonella. Each sample ( 10 g) was
homogenised and serial dilutions were inoculated on specific medium. Afterwards, incubations were
performed depending on the microorganism (Estevinho et al., 2012). Results were expressed as colonyforming
units per gram of dehydrated bee-pollen (CFU.g-1) for mesophilic, psychotrophs, Staphylococcus
aureus, total coliforms and yeasts and molds or as presence or absent for sulphite-reducing Clostridium
spores and Salmonella.
Description
Keywords
Bee-pollen Gamma-irradiation Microbiological parameters
Citation
Melo, Adriana A.; Estevinho, Leticia M.; Almeida-Muradian, Ligia (2014). Efect of gamma irradiation on the microbiological quality of dehydrated bee-pollen. In III International Symposion on Bee Products. Opatija, Croacia