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Tracking red blood cells flowing through a microchannel with a hyperbolic contraction: an automatic method

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The present chapter aims to assess the motion and deformation index of red blood cells (RBCs) flowing through a microchannel with a hyperbolic contraction using an image analysis based method. For this purpose, a microchannel containing a hyperbolic contraction was fabricated in polydimethylsiloxane by using a soft-lithography technique and the images were captured by a standard high-speed microscopy system. An automatic image processing and analyzing method has been developed in a MATLAB environment, not only to track both healthy and exposed RBCs motion but also to measure the deformation index along the microchannel. The keyhole model has proved to be a promising technique to track automatically healthy and exposed RBCs flowing in this kind of microchannels.

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Optical flow Bilateral filter Atomic regions Motion segmentation Deformation index

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Taboada, B.; Monteiro, Fernando C.; Lima, R. (2015). Tracking red blood cells flowing through a microchannel with a hyperbolic contraction: an automatic method. In Developments in Medical Image Processing and Computational Vision. Heidelberg: Springer, p. 105-119. ISBN 978-3-319-13406-2

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Springer Verlag

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