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Advisor(s)
Abstract(s)
The number of colonies in a culture is counted to calculate the concentration of
bacteria in the original broth; however, manual counting can be tedious, timeconsuming
and imprecise. Automation of colony counting has been of increasing
interest for many decades, and these methods have been shown to be more
consistent than manual counting. Significant limitations of many algorithms used
in automated systems are their inability to recognize overlapping colonies as
distinct and to count colonies on the plate boundary. This study proposes an
interactive semi-automated counting system and a fully automated counting
system using image processing methods which overcomes these problems. The
proposed systems are capable to reduce the manpower and time required for
counting colonies while taking account colonies both around the central area and
boundary areas of a Petri dish. The results obtained are compared with other
methods found in the literature.
Description
Keywords
Interactive method Count bacterial colonies
Citation
Ribeiro, João; Martins, Ramiro; Monteiro, Fernando (2015). Interactive/automated method to count bacterial colonies. In RECPAD 2015 - 21St Edition of the Portuguese Conference on Pattern Recognition. Faro