Advisor(s)
Abstract(s)
The understanding of the macromolecular properties of lignin requires information on its molecular weight distributions. Size exclusion chromatography using DMF/LiBr as the mobile phase and PS-DVB gels as the stationary phase is a widely used system. In this study, two different lignin samples, Indulin-AT and Alcell, were analysed under different experimental conditions. The effect of temperature (60 and 80 ºC), LiBr concentration (0.1 and 0.9%, w/w) and sample preparation conditions (lignin concentration and dissolution time prior to analysis) were analysed. The influence of temperature and ionic strength on a polystyrene-based calibration curve was also studied.
The two lignin samples have shown a distinct behaviour. Moreover, Alcell lignin in DMF/LiBr was found to be a viable model system to perform molecular weight investigations, particularly, those dealing with comparative studies with different molecular weight characterization techniques. With the addition of LiBr to DMF a unimodal MWD and an elution profile independent of sample concentration and used dissolution time was obtained. Moreover, a linear concentration dependence as a function of the UV response was observed.
Description
Keywords
Lignin SEC DMF-LiBr Molecular weight
Citation
Cateto, C.A.; Barreiro, M.F.; Belgacem, M.N.; Rodrigues, A.E. (2008). Size exclusion chromatography of lignin in N-N-dimethylformamide/lithium bromide. In 10th. European Workshop on Lignocellulosics and Pulp – EWLP. Stockholm, Sueden.
Publisher
Royal Institute of Technology