Advisor(s)
Abstract(s)
Lutein is a bioactive found in dark leafy vegetables that may be used as a nutraceutical agent in foodstuff and an
inhibitor of key enzymes of the human body such as those involved in the cholinergic system. However, its high
hydrophobicity leads to low bioavailability and must be overcome if lutein is to be added in foods. The objective
of this study was to evaluate the influence of nanoencapsulated lutein in the activity of the acetylcholinesterase
enzyme. The in vitro study was carried out using water in order to evaluate the impact of encapsulation on the
hydrophilicity of lutein. In vitro assays showed that lutein, both free and nanoencapsulated, presented a mixedtype
inhibition behavior, and encapsulated lutein was able to inhibit acetylcholinesterase activity even in an
aqueous medium. Inhibition was also showed by the in silico docking results which show that lutein interacted
with the pocket region of the enzyme.
Description
Keywords
Bioactive Cholinergic hypothesis Encapsulation Nanotechnology Nutraceutical
Citation
Grella Miranda, Cristiane; dos Santos, Priscila Dayane Freitas; do Prado Silva, Jéssica Thaís; Vitória Leimann, Fernanda; Ferreira Borges, Bianca; Miguel Abreu, Rui; Porto Ineu, Rafael; Hess Gonçalves, Odinei (2020). Influence of nanoencapsulated lutein on acetylcholinesterase activity: In vitro determination, kinetic parameters, and in silico docking simulations. Food Chemistry. ISSN 0308-8146. 307, p.