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Abstract(s)
Plants used in folk medicine have increased importance in the pharmaceutical field. Achillea
millefolium, also known as yarrow, is a traditional plant that can be found in Europe, Asia, North
Africa and North America [1, 2]. Yarrow was shown to have antitumour, antimicrobial, antiinflammatory
and antioxidant properties [3]. Indeed, several studies indicated that this plant has
antioxidant potential due to its chemical composition in phenolic acids and flavones [3].
The aim of this work was to study if an aqueous extract of A. millefolium presents cell growth
inhibitory activity in three human tumor cell lines: non-small cell lung cancer (NCI-H460), human
colorectal adenocarcinoma (HCT-15) and breast adenocarcinoma (MCF-7). Sulforhodamine B
assay was carried out to determine in which cells the extract was more potent. In addition, the
effect of the aqueous extract on NCI-H460 cell cycle profile and programmed cell death was
analyzed by flow cytometry following incubation with propidium iodide (PI) or Annexin V-FICT/PI
staining, respectively.
Results showed that A. millefolium extract inhibited the growth of the mentioned cell lines.
Moreover, further studies of this extract in NCI-H460 cells indicated that the reduction in cell
growth was due to alterations in cell cycle profile and in apoptosis. Future work will also include
analysis of protein expression by Western Blot to further unravel the mechanism of action of this
extract.
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Citation
Pereira, Joana M.; Peixoto, Vanessa; Teixeira, Alexandra; Sousa, Diana; Ferreira, Isabel C.F.R.; Vasconcelos, M. Helena (2017). Cell growth inhibitory effect of an aqueous extract of Achillea millefolium in human tumor cell lines. In 10.º Encontro de Jovens Investigadores da U.PORTO. ISBN 978-989-746-117-0