| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 20.75 MB | Adobe PDF |
Advisor(s)
Abstract(s)
Ganoderma lucidum is a mycological source rich in several bioactive compounds. Among their
constituents, triterpenes have been widely reported to be responsible for some of G. lucidum bioactive
properties [1]. It has been used, since ancestral times, due to their medicinal and nutraceutical
properties. In this context, the achievement of extracts enriched in target compounds, and obtained at
optimized extraction conditions, is a relevant field of research where the use of statistical tools, such as
response surface methodology (RSM), is becoming increasingly important.
In the present study, the extraction of triterpenes from G. lucidum was optimized by using maceration
(ME) and ultrasound assisted extraction (UAE), and compared with Soxhlet extraction (SE). The SE
technique was considered effective by applying 7 cycles while to optimize ME and UAE, RSM was
applied using a circumscribed central composite design with three independent variables (time, ethanol
content, temperature (ME) or power (UAE)). The extraction yield and content in triterpenes were
maximized and the extracts obtained under the optimal conditions characterized in terms of individual
triterpenoids by HPLC-DAD-ESI/MS analysis.
With the SE technique, a positive linear dependency was achieved for the extraction yield while an
asymptotic-decreasing behavior was observed for triterpenes’ content. The conditions maximizing
responses were: 78.9 min, 90ºC and 62.5% ethanol and 40 min, 100 W and 89.5 % ethanol for ME and
UAE, respectively. The later was the most effective, yielding 4.9±0.6% and 435.6±21.1 mg equiv. ursolic
acid/g extract whereas the first yielded 5.2±0.6% and 285.7±31.2 mg equiv. ursolic acid/g extract. Under
the optimum conditions, HPLC-DAD-ESI/MSn analysis confirmed the presence of a total of 24, 26 and 28
triterpenes in the extracts obtained by ME, UAE and SE, respectively. These results show the potential of
G. lucidum as a source of bioactive compounds pointing out for their use in several industrial
applications.
Description
Keywords
Citation
Taofiq, Oludemi; Barros, Lillian; Prieto, Miguel; Barreiro, M.F.; Ferreira, Isabel C.F.R. (2017). Optimization of the extraction of triterpenes from Ganoderma lucidum. In 10º Encontro Nacional de Cromatografia. Bragança. ISBN 978-972-745-234-7
Publisher
Instituto Politécnico de Bragança