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Advisor(s)
Abstract(s)
Three different HPLC detection systems were compared for the determination of tocopherols and
tocotrienols in olive oil: fluorescence and diode array connected in series, ultraviolet, and evaporative
light scattering. The best results were obtained with the fluorescence detector, which was successfully
applied in the quantification of tocopherols and tocotrienols in 18 samples of Portuguese olive oils.
To support the validity of the method, the parameters evaluated were linearity, detection limits,
repeatability, and recovery. All of the studied samples showed similar qualitative profiles with six
identified compounds: R-T, -T, Îł-T, ÎŽ-T, R-T3, and Îł-T3. R-Tocopherol (R-T) was the main vitamin
E isomer in all samples ranging from 93 to 260 mg/kg. The total tocopherols and tocotrienols ranged
from 100 to 270 mg/kg. Geographic origin did not seem to influence the tocopherol and tocotrienol
composition of the olive oils under evaluation.
Description
Keywords
Tocopherols Tocotrienols Olive oil NP-HPLC
Citation
Cunha, S.C.; Amaral, J.S.; Fernandes, J.O.; Oliveira, M.B.P.P. (2006). Quantification of tocopherols and tocotrienols in Portuguese olive oils using HPLC with three different detection systems. Journal of Agricultural and Food Chemistry. ISSN 0021-8561. 54:9, p.3351â3356
Publisher
ACS