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Abstract(s)
Fragaria vesca L. is mainly consumed and appreciated by its sweet small fruits. However, the vegetative
parts have been described as important sources of macro and micronutrients as also phenolic
compounds (procyanidins and hydroxycinnamoyl and ellagic acid derivatives) [1,2]. The growing
demand for natural products with nutritional value and, simultaneously, bioactive properties requires
innovation in how to obtain these products, namely protecting the wild populations from where they are
collected avoiding direct competition with human food stuff cultures [3]. Cell and tissue culture emerges
as viable technique to produce secondary metabolites, being a sustainable and ecological alternative to
obtain bioactive compounds that can be applied in pharmaceutical/medical and/or food industry
(endorsed by FAO) [5]. Herein, the vegetative parts of F. vesca were obtained by in vitro culture and
characterized in terms of macronutrients (AOAC methods), fatty acids (GC-FID), sugars (HPLC-RI),
organic acids (HPLC-DAD), tocopherols (HPLC-Fluorescence), and phenolic compounds (HPLC-DADESI/
MS). The antioxidant activity was also evaluated. The studies were carried out with lyophilized
material and hydromethanolic and aqueous (infusions and decoctions) extracts. In order to establish the
in vitro culture, a four week interval was followed for micropropagation In each one, the vegetative parts
were collected and characterized, presenting higher contents of proteins, fatty acids, sugars, organic
acids (including ascorbic acid) and tocopherols (mainly α-tocopherol) than the samples obtained from
wild vegetative parts collected in field and previously studied by our research group [2]. Furthermore, the
hydromethanolic extracts of the obtained in vitro samples also revealed higher antioxidant activity that the
F. vesca samples collected in field [4]. On the contrary and despite the similarity between the phenolic
profile of both samples, lower concentrations were detected in the in vitro cultured F. vesca. Further
studies are required to understand how to increase the concentration of phenolic compounds by
elicitation of the in vitro culture. Within this work, it was intended to explore the in vitro culture as a
biotechnological tool for the obtention of high value phytochemicals with application in different industrial
sectors.
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Pedagogical Context
Citation
Dias, Maria Inês; Barros, Lillian; Sousa, Maria João R.P.; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R. (2017). Chromatographic analysis of nutritional and bioactive compounds in vegetative parts of Fragaria vesca L. obtained by in vitro culture. In 10º Encontro Nacional de Cromatografia. Bragança. ISBN 978-972-745-234-7