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Advisor(s)
Abstract(s)
Response surface methodology (RSM) was employed for the first time to optimize high pressure extraction (HPE) conditions of bioactive compounds from pansies, namely: pressure (X1: 0–500 MPa), time (X2: 5–15 min) and ethanol concentration (X3: 0–100%). Consistent fittings using second-order polynomial models
were obtained for flavonoids, tannins, anthocyanins, total reducing capacity (TRC) and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity. The optimum extraction conditions based on combination responses for TRC, tannins and anthocyanins were: X1 = 384 MPa, X2 = 15 min and X3 = 35% (v/v) ethanol, shortening the extraction time when compared to the classic method of stirring (approx. 24 h). When the optimum extraction conditions were applied, 65.1 mg of TRC, 42.8 mg of tannins and 56.15 mg of anthocyanins/g dried flower were obtained. Thus, HPE has shown to be a promising technique to extract bioactive compounds from pansies, by reducing the extraction time and by using green solvents (ethanol and water), for application in diverse industrial fields.
Description
Keywords
Pansies flowers High pressure extraction Antioxidant activity Bioactive compounds Response surface methodology
Citation
Fernandes, Luana; Casal, Susana; Pereira, J.A.; Ramalhosa, Elsa; Saraiva, Jorge (2017). Optimization of high pressure bioactive compounds extraction from pansies (Viola × wittrockiana) by response surface methodology. High Pressure Research. ISSN 0895-7959. 37:3, p. 415-429