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Abstract(s)
Agaricus bisporus L. is the most consumed mushroom worldwide exhibiting a high
content of ergosterol in its sterol’s fraction (~90%). Some studies have demonstrated
the health benefits of this molecule related to its antioxidant, antitumor and also
hypocholesterolemic effects, similar to the ones exhibited by phytosterols. Functional
foods containing phytosterols, or their derivatives, can be found at commercial level
bearing the European Food Safety Authority approved health claim “able to lower
cholesterol levels in serum”. Therefore, this work aims to present the use of
mycosterols as an innovative alternative to phytosterols. A. bisporus bioresidues
obtained from mushroom’s industry were used to obtain mycosterols by ultrasound
assisted extraction. Pure ergosterol and the obtained mycosterol extracts were added
to dairy beverages, in a similar amount to the one used in the commercial ones
incorporated with phytosterols. All the beverages were analysed immediately (T0) and
after seven days of storage at 4ºC (T1) regarding nutritional parameters, antioxidant
activity and cytotoxic properties against human tumor cell lines and a non‐tumor
porcine liver primary culture. The beverages functionalized with the mycosterols
extract revealed an antioxidant activity similar to the commercial beverages with
phytosterols. However, beverages with pure ergosterol revealed twice this antioxidant
activity. Moreover, only the samples functionalized with pure ergosterol or the
mycosterols extract have shown an increase in the antioxidant activity from T0 to T1,
meaning that the compound/extracts were able to protect the dairy drink from
oxidation, increasing the product shelf life. Samples with pure ergosterol also revealed
the highest cytotoxicity for tumor cell lines, while the beverages with phytosterols
showed the lowest activity with no significant differences between T0 and T1. All the
samples revealed a similar nutritional composition, and none of them revealed toxicity
for normal cells. Studies on the hypocholesterolemic effects of the developed
beverages are being carried out.
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Citation
Heleno, Sandrina A.; Rudke, Adenilson; Calhelha, Ricardo C.; Barros, Lillian; Gonçalves, Odinei H.; Rodrigues, A.E.; Barreiro, M.F.; Ferreira, Isabel C.F.R. (2016). Bioactive properties of dairy beverages functionalized with pure ergosterol and mycosterol extracts: a comparative study with phytosterol counterparts. In I International Conference on Research for Sustainable Development in Mountain Regions: Book of Abstracts. Bragança. ISBN 978‐972‐745‐214‐9