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Abstract(s)
Saffron (Crocus sativus L.) flower is composed of six purple tepals, three yellow stamens and a white filiform style ending
in a stigma with three threads, which only represents less than 10% (w/w) of the flower weight. Nevertheless, saffron is
cultivated for the stigma of its flowers which, after being dried, is the most valued spice [1]. For each kg of this spice,
about 63 kg of floral bio-residues are produced, which so far are not exploited, being usually thrown away. However, the
floral bio-residues were reported as having high phenolic content and bioactive properties, such as antioxidant,
antityrosinase, antidepressant, antinociceptive, anti-inflammatory, antifungal and arterial pressure reducer activities [2]. In
view of the reported bioactivity, it is important to study the cytotoxic effects of saffron [3]. Furthermore, it is a wellknown
fact that the effectiveness of bioactive compounds extraction from plants, as well as their corresponding activity, is
highly dependent on factors such as different types of solvent, solvent-to-solid ratios and specially the solvent polarity [4].
In the present work, the antioxidant activity of different polar extracts (ethanol, ethanol:water 1:1 v/v, and water) of saffron
was evaluated using different in vitro assays (2,2-diphenyl-1-picrylhydrazyl radical-scavenging activity, ferric reducing
reducing power and inhibition of β-carotene bleaching assay). In addition, the cytotoxicity of the extracts was also
evaluated in Caco-2 (ATCC HTB-37TM) cultures by using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium
bromide) assay. All samples proved to have antioxidant activity, despite the higher effectiveness of the hydroalcoholic
extract. Up to the assayed concentrations, none of the extracts showed cytotoxicity against Caco-2 cell lines. Accordingly,
saffron flowers might be used in different applications such as the development of food supplements or pharmaceutic related products.
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Citation
Barreira, João C.M.; Rebelo, José Miguel; Costa, Carla; Costa, Anabela S.G.; Ferreira, Isabel C.F.R.; Teixeira, João; Oliveira, M.B.P.P. (2014). Antioxidant activity and cytotoxic effects of polar extracts from saffron (Crocus sativus L) flowers. In 12º Encontro de Química dos Alimentos. Lisboa. ISBN 978-989-98541-5-4