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Advisor(s)
Abstract(s)
Artichoke (A), borututu (B) and milk thistle (M) are included in several supplements to provide beneficial effects. Different formulations (infusions, pills and syrups), as also different proportions of A, B and M (1:1:1, 2:1:1, 1:2:1, 1:1:2) within each formulation were assayed to optimize the desired benefits. The antioxidant activity, anti-hepatocellular carcinoma activity, hepatotoxicity and bioactive compounds contents were evaluated. Syrups tended to be the formulation with highest antioxidant activity and total phenolics and flavonoids content; otherwise, pills were the worst formulation. In what concerns A:B:M ratios, the results did not reveal so pronounced differences. None of the assayed mixtures resulted to be toxic (up to the maximum assayed dose) for liver primary cells (PLP2), but some samples, especially infusions, showed toxicity for the hepatocellular carcinoma cell line (HepG2). With no exception, the mixtures for all formulations gave synergistic effects in antioxidant activity, when compared to the activity of single plants.
Description
Keywords
Dietary supplements Formulation Compositional mixture Bioactivity Citotoxicity Synergism
Pedagogical Context
Citation
Pereira, Carla; Barreira, João C.M.; Calhelha, Ricardo C.; Queiroz, Maria João R.P.; Barros, Lillian; Ferreira, Isabel C.F.R. (2014). New insights into the effects of formulation type and compositional mixtures over the antioxidant and cytotoxic activities of dietary supplements based-on hepatoprotective plants. Food & Function. ISSN 2042-6496. 5:9, p. 2052-2060
Publisher
Royal Society of Chemistry