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Advisor(s)
Abstract(s)
The aim of this work was to study the effect of recombinant human erythropoietin (rhEPO) the rapy
on haemorheological parameters, by using a rat model of chronic renal failure (CRF) induced by
partial (3/4) nephrectomy.
The study used adult male Wistar rats and was performed in three groups: a control one (n=6) and
in two groups with induced chronic renal failure (n=9), being one of them submitted to rhEPO the rapy
(n=4). Blood samples from the control group were collected at the beginning and at the end of the
experimental procedure and from CRF rats at 3, 5, 8, 12 and 15 weeks after surgical partial nephrectomy.
Haemorheology and renal function were evaluated.
Three weeks after the 3/4 nephrectomy, a statistically significant increase in serum urea and creatinine
concentrations were found. This increase in renal function markers remained high along the 12
weeks of experimental procedure.
Comparing with controls, rhEPO treated rat have showed a statistically significant progressive
increase in haemoglobin (Hb), haematocrit (Ht), red blood cells (RBC) count, mean cell volume (MCV), mean cell Hb (MCH) and red cell distribution width (RDW), showing at 12 weeks an inverse
change, though still presenting significant higher values; a decrease in platelet counts, during the first
9 weeks of rhEPO therapy. When comparing haemorheological data from non-treated CRF and controls,
we found only a trend to increased MCV and MCH values and a decrease in reticulocyte count.
Comparing the two groups of CRF rats, we found that rhEPO treated rats presented significantly
higher values in RBC, Hb, Ht and RDW. In both groups of CRF rats, at five weeks, there was a decrease
in their values, showing at the end a significantly lower value when compared to controls. No
consistent alterations were found in white blood cells in CRF rats, with or without rhEPO therapy.
Partial nephrectomy seems to be a suitable methodology to induce CRF in rats and to study erythropoiesis
biology. The rhEPO therapy is associated with an increased erythropoietic stimulation and
a decrease in platelet count.
Description
Keywords
rhEPO Erythropoietin Renal failure Rat model Haemorheology
Citation
Rocha, Susana; Castro, Elisabeth; Miranda, Vasco; Faria, Maria Sameiro; Loureiro, Alfredo; Lemos, Edite Teixeira de; Parada, Belmiro; Figueiredo, Arnaldo; Quintanilha, Alexandre; Teixeira, Frederico; Belo; Luís, Santos-Silva, Alice (2008). Haemorheological changes during recombinante human erythropoietin therapy in a rat model of renal failure induced by partial nephrectomy. In Actas de Bioquímica. Lisboa. p. 125-129. ISBN 972-590-076-6