Development of an electrochemical aptasensor for the detection of human osteopontin

Meirinho, Sofia G.; Dias, L.G.; Peres, António M.; Rodrigues, Lígia R.

http://hdl.handle.net/10198/11810

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Abstract(s)

Electrochemical aptasensors, an emerging technology, enables the detection of protein biomarkers, which may be indicative of tumour activity. Osteopontin is a protein present in body fluids, being a possible biomarker since its overexpression has been related with breast cancer progression. An RNA aptamer, described in the literature, with affinity for human osteopontin, was synthetized, immobilized in a microelectrode gold surface and used for development electrochemical aptasensor for human OPN detection in standard solutions. Cyclic voltammetry results showed that this aptasensor allowed detecting human osteopontin with a detection limit of 8 nM, showing a satisfactory selectivity towards the target in the presence of others proteins, except for thrombin.

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http://hdl.handle.net/10198/11810

Citation

Meirinho, S.G.; Dias, L.G.; Peres, António M.; Rodrigues, Lígia R. (2014). Development of an electrochemical aptasensor for the detection of human osteopontin. In XXVIII EUROSENSORS e Procedia Engineering. 87, p. 316-319