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Prunus avium L. (cereja doce) Ă© um dos frutos de clima temperado mais popular, sendo altamente apreciado pelos consumidores e extensamente estudado pela comunidade cientĂfica. Ao contrĂĄrio dos frutos que tĂȘm sido amplamente estudados, nada se sabe sobre a composição quĂmica e propriedades bioativas dos pedĂșnculos de P. avium, apesar do uso na medicina tradicional de infusĂ”es e decocçÔes preparadas a partir dessas partes. Portanto, o objetivo deste estudo foi caracterizar frutos e pedĂșnculos de cereja doce quanto Ă sua composição nutricional, fitoquĂmicos individuais e propriedades bioativas. Foram estabelecidos os perfis cromatogrĂĄficos em açĂșcares, ĂĄcidos orgĂąnicos, ĂĄcidos gordos, tocoferĂłis e compostos fenĂłlicos. Todas as preparaçÔes (extratos metanol/ĂĄgua 80:20 v/v, infusĂ”es e decocçÔes) obtidas utilizando pedĂșnculos revelaram maior potencial antioxidante do que o extrato metanol/ĂĄgua obtido a partir dos frutos, o que certamente estĂĄ relacionado com a maior concentração de compostos fenĂłlicos (ĂĄcidos fenĂłlicos e flavonoides) encontrada nos pedĂșnculos, em comparação com os frutos. O extrato metanol/ĂĄgua obtido a partir dos frutos foi o Ășnico a revelar potencial antitumoral, com especificidade para a linha celular humana HCT-15 (carcinoma do cĂłlon) (GI50~74 ïg/mL). Este resultado pode estar relacionado com a presença de antocianinas, que sĂł foram encontradas nos frutos e nĂŁo nos pedĂșnculos. Nenhuma das preparaçÔes testadas mostrou hepatotoxicidade contra cĂ©lulas normais. No geral, o presente estudo apresenta resultados inovadores em relação Ă caracterização quĂmica e propriedades bioativas de pedĂșnculos de cereja doce, e confirmou as caracterĂsticas nutricionais e antioxidantes dos seus frutos.
Prunus avium L. (sweet cherry) is one of the most popular climate fruits, being highly appreciated by consumers and studied by the scientific community. In opposition to the widely studied fruits and despite the traditional medicinal use of infusions and decoctions prepared from P. avium stems, nothing is known about their chemical composition and bioactive properties. Therefore, the aim of this study was to characterise sweet cherry fruits and stems regarding nutritional composition, individual phytochemicals and bioactive properties. The chromatographic profiles in sugars, organic acids, fatty acids, tocopherols and phenolic compounds were established. All the preparations (methanol/water 80:20 v/v extracts, infusions and decoctions) obtained using stems revealed higher antioxidant potential than the tested methanol/water extract from fruits, which is certainly related with the higher phenolic compounds (phenolic acids and flavonoids) concentration found in stems, in comparison with fruits. The fruits methanol/water extract was the only one showing antitumor potential, revealing selectivity against HCT-15 (colon carcinoma) (GI50~74 ïg/mL). This could be related with the presence of anthocyanins that were only found in fruits and not in stems. None of the tested preparations have shown hepatotoxicity against normal primary cells. Overall, the present study reports innovative results regarding chemical characterization and bioactive properties of sweet cherry stems, and confirmed the nutritional and antioxidant characteristics of their fruits.
Prunus avium L. (sweet cherry) is one of the most popular climate fruits, being highly appreciated by consumers and studied by the scientific community. In opposition to the widely studied fruits and despite the traditional medicinal use of infusions and decoctions prepared from P. avium stems, nothing is known about their chemical composition and bioactive properties. Therefore, the aim of this study was to characterise sweet cherry fruits and stems regarding nutritional composition, individual phytochemicals and bioactive properties. The chromatographic profiles in sugars, organic acids, fatty acids, tocopherols and phenolic compounds were established. All the preparations (methanol/water 80:20 v/v extracts, infusions and decoctions) obtained using stems revealed higher antioxidant potential than the tested methanol/water extract from fruits, which is certainly related with the higher phenolic compounds (phenolic acids and flavonoids) concentration found in stems, in comparison with fruits. The fruits methanol/water extract was the only one showing antitumor potential, revealing selectivity against HCT-15 (colon carcinoma) (GI50~74 ïg/mL). This could be related with the presence of anthocyanins that were only found in fruits and not in stems. None of the tested preparations have shown hepatotoxicity against normal primary cells. Overall, the present study reports innovative results regarding chemical characterization and bioactive properties of sweet cherry stems, and confirmed the nutritional and antioxidant characteristics of their fruits.