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Advisor(s)
Abstract(s)
Species identification in meat products has grown in interest in recent years since these foodstuffs are susceptible
targets for fraudulent labelling. In this work, a real-time PCR approach based on SYBR Green dye
was proposed for the quantitative detection of pork meat in processed meat products. For the development
of the method, binary meat mixtures containing known amounts of pork meat in poultry meat were used to
obtain a normalised calibration model from 0.1 to 25% with high linear correlation and PCR efficiency. The
method revealed high specificity by melting curve analysis, being successfully validated through its application
to blind meat mixtures, which confirmed its adequacy for pork meat determination. The fully applicability
of the method was further demonstrated in commercial meat products, allowing verification of labelling
compliance and identification of meat species in processed foods.
Description
Keywords
Quantitative real-time PCR SYBR green Authentication Species identification Pork meat Poultry meat
Citation
Soares, S.; Amaral, J.S.; Oliveira, M.B.P.P.; Mafra, I. (2013). A SYBR Green real-time PCR assay to detect and quantify pork meat in processed poultry meat products. Meat Science. ISSN 0309-1740. 94, p.115-120
Publisher
Elsevier